Enzyme found to disrupt Ebola replication

first_imgApr 15, 2005 (CIDRAP News) – The Ebola virus has yielded an important behavioral clue that could lead to a treatment for the incurable infection that kills 50% to 90% of its victims, researchers have announced.Enzyme-inhibiting chemicals have stalled the virus’ reproduction in laboratory-grown cells, reports senior author James M. Cunningham, MD, of Brigham and Women’s Hospital and Harvard Medical School in Boston, in an article published online yesterday in Science. The research was partly supported by the National Institute of Allergy and Infectiuos Diseases (NIAID), part of the National Institutes of Health (NIH).The researchers found two cellular enzymes the Ebola virus depends upon to reproduce. When they are blocked, the virus’ infectivity drops a great deal, according to a news release from NIAID.The Ebola virus uses the cellular enzymes to cut up viral surface proteins. Once the protein is split, the virus can multiply.Scientists applied broad-spectrum enzyme inhibitors to mammal cells before exposing them to Ebola. When an enzyme called cathepsin B was suppressed, the infectivity of the Ebola virus “dropped to near zero,” the news release said.The other enzyme, cathepsin L, played a helping role in Ebola multiplication, researchers found. Drugs that inhibit cathepsins are already being developed to fight cancer.The findings may be significant because Ebola, like its fellow filovirus Marburg, is part of a family of viruses that strike relatively rarely but can cause severe, often fatal hemorrhagic fevers. An ongoing Marburg outbreak in the Uige province of Angola had as of yesterday sickened 235 people, killing 215.”Finding medical countermeasures for viral hemorrhagic fevers is a global public health priority because not only do these diseases occur naturally, but they also have the potential to be unleashed by bioterrorists,” says NIH Director Elias A. Zerhouni, MD.The research illuminates the mechanism the Ebola virus uses to insinuate itself into cells, said NIAID Director Anthony S. Fauci, MD. “These findings raise the possibility of a broad-spectrum antiviral therapy that could be effective against multiple hemorrhagic fever viruses.Chandran K, Sullivan NJ, Felbor U, et al. Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection. Science 2005 (published online Apr 14) http://www.sciencemag.org/cgi/content/abstract/1110656v1See also: NIH news release at http://www.nih.gov/news/pr/apr2005/niaid-14.htm.last_img read more

Unai Emery told to drop Petr Cech for Arsenal’s Europa League final v Chelsea

first_imgArsenal’s Bernd Leno should start against Chelsea rather than Petr Cech, according to Danny Mills (Picture: Getty)Unai Emery should start Bernd Leno over Petr Cech in Arsenal’s Europa League final against Chelsea, according to Danny Mills.Cech has been a regular for the Gunners in the Europa League this season but Mills says Emery should put his faith in Leno for next week’s final in Baku.Arsenal spent £22.5million on Leno last summer and the German goalkeeper has impressed in his first season at the Emirates.Cech has been tipped to take up a newly-created sporting director role at his former club Chelsea this summer and Mills says this is another reason why he should not start the Europa League final.AdvertisementAdvertisementADVERTISEMENTMore: FootballRio Ferdinand urges Ole Gunnar Solskjaer to drop Manchester United starChelsea defender Fikayo Tomori reveals why he made U-turn over transfer deadline day moveMikel Arteta rates Thomas Partey’s chances of making his Arsenal debut vs Man City‘You start to call into question the player’s integrity, his ability to perform,’ former Premier League star Mills told talkSPORT.‘And I don’t think that’s in question, but the problem is if he makes a mistake, if something happens in that game.‘Go back to the start of the season where I think they played against Manchester City and he tried to play out from the back and he gave it to some of the Man City players.‘Imagine if he did that again… I just think you’re putting him under enormous pressure, it puts himself under incredible pressure. Comment Advertisement Metro Sport ReporterFriday 24 May 2019 7:59 amShare this article via facebookShare this article via twitterShare this article via messengerShare this with Share this article via emailShare this article via flipboardCopy link397Shares Emery speaks to press ahead of the Europa League finalTo view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Play VideoLoaded: 0%0:00Progress: 0%PlayMuteCurrent Time 0:00/Duration Time 24:21FullscreenEmery speaks to press ahead of the Europa League finalhttps://metro.co.uk/video/emery-speaks-press-ahead-europa-league-final-1929672/This is a modal window.Beginning of dialog window. Escape will cancel and close the window.TextColorWhiteBlackRedGreenBlueYellowMagentaCyanTransparencyOpaqueSemi-TransparentBackgroundColorBlackWhiteRedGreenBlueYellowMagentaCyanTransparencyOpaqueSemi-TransparentTransparentWindowColorBlackWhiteRedGreenBlueYellowMagentaCyanTransparencyTransparentSemi-TransparentOpaqueFont Size50%75%100%125%150%175%200%300%400%Text Edge StyleNoneRaisedDepressedUniformDropshadowFont FamilyProportional Sans-SerifMonospace Sans-SerifProportional SerifMonospace SerifCasualScriptSmall CapsReset restore all settings to the default valuesDoneClose Modal DialogEnd of dialog window.‘If I was Arsenal, I’m not sure I’d want that. If he makes a mistake, if something happens, there will always be that doubt in people’s minds.’Ex-England defender Mills added: ‘If I was Arsenal, and they must know what he’s doing and where he’s going at the end of the season, if he is going to go to Chelsea as we believe, I wouldn’t play him.‘He can still walk around the pitch and clap all the fans. But I would not play him in that situation.’Cech, meanwhile, has dismissed reports suggesting he has already agreed a deal with Chelsea to return to Stamford Bridge when the season finishes.More: FootballBruno Fernandes responds to Man Utd bust-up rumours with Ole Gunnar SolskjaerNew Manchester United signing Facundo Pellistri responds to Edinson Cavani praiseArsenal flop Denis Suarez delivers verdict on Thomas Partey and Lucas Torreira movesHe wrote on Twitter: ‘Despite the news today as I had already said to everybody before, I’ll make a decision about my future after the last game.‘Now my sole focus is to win the Europa League with Arsenal.’Arsenal finished the 2018-19 Premier League season in fifth place, one point behind north London rivals Tottenham and two behind Chelsea.The Gunners will qualify for next season’s Champions League if they beat domestic rivals Chelsea in Baku.MORE: Jose Mourinho backs ‘fantastic’ Eden Hazard to join Real Madrid Advertisement Unai Emery told to drop Petr Cech for Arsenal’s Europa League final v Chelsealast_img read more

Video: Ndidi, Vardy opt for boxing gloves ahead of fight for top four place

first_imgSuper Eagles star Wilfred Ndidi and his Leicester teammate Jamie Vardy opted for the boxing gloves as the Foxes showed they still hold plenty of fighting spirit in their push for a Champions League spot. The duo were filmed locking horns in a lighthearted sparring session at Leicester’s training base, with both donning boxing gloves to hone their speed and agility heading into the latter stages of the season. And after the pair traded a series of punches, Vardy floored Ndidi with a sharp left hook – much to the amusement of his team-mate. Brendan Rodgers’ high-flyers appeared set to challenge Manchester City for second spot after a formidable run of form, but their momentum has halted after collecting one victory in their last five league games. A limp defeat at struggling Norwich was compounded by Kelechi Iheanacho’s stellar strike being ruled out by VAR for handball, before Jamal Lewis’; goal dealt the visitors another hammer blow on 70 minutes. The Foxes boss demanded that his side now step up in front of goal, and frontman Vardy, who missed the trip to Carrow Road, was snapped being put through his paces by midfield enforcer Ndidi. The humorous video was posted on Ndidi’s social media channels, and captioned ‘We will back stronger’. And in the final few seconds, Vardy connected with a lightning jab and sent the former Genk star tumbling. Vardy raced out of the shot after connecting with a sharp left hook and flooring his team-mate Read Also: Victor Moses suffers injury setback, set to miss Inter’s matches The Premier League’s joint-top scorer then raced out of the shot after dealing the damage, and struggled to contain his laughter. Leicester will look to haul their top four challenge back on track against Aston Villa next Monday. FacebookTwitterWhatsAppEmail分享 center_img Loading…last_img read more

Cech issues Saints warning

first_imgPetr Cech has warned Chelsea that Saturday’s opponents Southampton are the most dangerous they have been all season following the arrival of new manager Mauricio Pochettino. Chelsea thumped Southampton 5-1 at St Mary’s Stadium in the FA Cup in January but less than two weeks later they blew a two-goal lead as the Saints claimed a point at Stamford Bridge. That proved to be Nigel Adkins’ last day in the charge of Southampton and Cech has been impressed by the improvements Pochettino has made since his arrival. “In football, everything is different – you can have a first half that is completely different from the second half,” Cech told cfc.com Press Associationcenter_img “In the away game against Southampton we were very effective, taking our chances, and then at home we had a very good half – but the last half an hour of the game we allowed them to come back into it. “You cannot do that in the Premier League. “The teams are strong enough to grab the chance if you give it to them – everyone in the Premier League has that quality and will take it if you let them. “They changed manager with Pochettino and they are more attacking, more organised with very good pressing which makes them more aggressive – and, in turn, more confident because of the success they’ve been having.” John Terry is available to manager Rafael Benitez – but his selection will be made with one eye on Monday’s FA Cup quarter-final replay against Manchester United. Benitez said it is “impossible” for players to peak twice in 48 hours so some rotation of the side is inevitable and Terry, after a season disrupted by a knee injury, is only likely to start one of them. Chelsea’s interim manager reported a clean bill of health following the international break and he is hopeful that Ramires (Achilles) and Gary Cahill (knee) could be fit for Monday. last_img read more

Gabby Agbonlahor red card rescinded

first_img But manager Paul Lambert was delighted at Agbonlahor’s reprieve after he was dismissed – for the first time in his career – by referee Lee Mason. He said: “It is great for us. I said at the time I didn’t think it was a red card and everyone’s assumption was the same. “Ex-referees have come out and said it wasn’t. “So your own common sense told you it wasn’t a red card. “Gabby is playing really well at the minute so we hoped it would be rescinded. “We are still without a few – Westy (Ashley Westwood), Nathan (Baker), (Philippe) Senderos and (Kieran) Richardson – so we didn’t want another one out. “Not one of your big players.” The red was Villa’s second in two games after Richardson was dismissed in their 1-0 defeat at West Brom. Richardson is still suspended but Villa have Alan Hutton back after a ban for their trip to the Liberty Stadium while Tom Cleverley is also available following his fifth yellow card of the season. The striker was dismissed in the second half of Villa’s 1-1 draw with United on Saturday after a 50-50 tackle with former Villa team-mate Ashley Young, but is now available to face Swansea on Boxing Day. Villa, though, have been charged by the Football Association with “failing to ensure that its players conducted themselves in an orderly fashion” during the game and have until 6pm on December 30 to respond. Press Associationcenter_img Gabriel Agbonlahor’s red card against Manchester United has been overturned after Aston Villa won their appeal.last_img read more

FSU alerts the public about ‘dangerous situation’ near campus

first_imgNo other information is available at this time. Authorities at Florida State University have sent out an alert about a possibly dangerous situation near their campus.The alert was sent around 12pm Monday, during a police search for a possibly armed subject near Stadium Drive.The male subject is said to be traveling on foot, wearing shorts and no shirt.Law Enforcement Officers are in pursuit of a male subject wearing shorts and no shirt, traveling on foot in the direction of Stadium Drive from Jackson Bluff Road. Suspect potentially armed with a hand gun. Continue to Shelter-in-Place.More information to follow.— FSU Alert (@FSUAlert) June 8, 2020last_img